Reproducible and scalable purification of extracellular vesicles using combined bind-elute and size exclusion chromatography
نویسندگان
چکیده
منابع مشابه
Single-step isolation of extracellular vesicles by size-exclusion chromatography
BACKGROUND Isolation of extracellular vesicles from plasma is a challenge due to the presence of proteins and lipoproteins. Isolation of vesicles using differential centrifugation or density-gradient ultracentrifugation results in co-isolation of contaminants such as protein aggregates and incomplete separation of vesicles from lipoproteins, respectively. AIM To develop a single-step protocol...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/s41598-017-10646-x